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These are the fundamental concepts and rules that you need to know about laser, eye and general safety in the James R. Macdonald Lab. You should also see our main safety pages for more in-depth information on these topics, and when ready, take our exam.


Part 1: Laser Injuries

The Eye:

The eye is the most sensitive body organ to laser injury.  Because the eye is a light receptor and lasers produce light of extreme brightness, exposure of the eye to laser light can cause serious and lasting damage.  Before going on to eye-damage mechanisms, lets review the structure of the eye.

Parts of the human eye

The cornea is the transparent layer of tissue covering the eye.  Damage to the outer cornea may be uncomfortable (like a gritty feeling) or painful, but will usually heal quickly.  Damage to deeper layers of the cornea may cause permanent injury.

The lens focuses light to form images onto the retina.  Over time, the lens becomes less pliable, making it more difficult to focus on near objects.  With age, the lens also becomes cloudy and eventually opacifies.  This is known as a cataract.  Every lens develops cataract eventually.

The part of the eye that provides the most acute vision is the fovea centralis (also called the macula lutea).  This is a relatively small area of the retina (3 to 4%) that provides the most detailed and acute vision as well as color perception.  This is why eyes move when you read or when you look as something; the image has to be focused on the fovea for detailed perception. The balance of the retina can perceive light and movement, but not detailed images (peripheral vision).

If a laser burn occurs on the fovea, most fine (reading and working) vision may be lost in an instant.  If a laser burn occurs in the peripheral vision it may produce little or no effect on fine vision.  Repeated retinal burns can lead to blindness.

Fortunately the eye has a self-defense mechanism -- the blink or aversion response.  When a bright light hits the eye, the eye tends to blink or turn away from the light source (aversion) within a quarter of a second.  This may defend the eye from damage where lower power lasers are involved, but cannot help where higher power lasers are concerned.  With high power lasers, the damage can occur in less time than a quarter of a second.

Symptoms of a laser burn in the eye include a headache shortly after exposure, excessive watering of the eyes, and sudden appearance of floaters in your vision.  Floaters are those swirling distortions that occur randomly in normal vision most often after a blink or when eyes have been closed for a couple of seconds.  Floaters are caused by dead cell tissues that detach from the retina and choroid and float in the vitreous humor.  Ophthalmologists often dismiss minor laser injuries as floaters due to the very difficult task of detecting minor retinal injuries.  Minor corneal burns cause a gritty feeling, like sand in the eye.

Several factors determine the degree of injury to the eye from laser light:

  • Pupil size - The shrinking of pupil diameter reduces the amount of total energy delivered to the retinal surface.  Pupil size ranges from a 2 mm diameter in bright sun to an 8 mm diameter in darkness (night vision).
  • Degree of pigmentation - More pigment (melanin) results in more heat absorption.
  • Size of retinal image - The larger the size, the greater the damage because temperature equilibrium must be achieved to do damage.  The rate of equilibrium formation is determined by the size of the image.
  • Pulse duration - The shorter the time (ns versus ms), the greater the chance of injury.
  • Pulse repetition rate - The faster the rate, the less chance for heat dissipation and recovery.
  • Wavelength - determines where the energy deposits and how much gets through the ocular media.

Eye Absorption Site vs. Wavelength

Different wavelengths of light are absorbed in different parts of the eye. Visible light reaches the retina for image perception.  Other wavelengths interact with eye tissue differently. Where the laser energy will be absorbed in the eye depends on the light’s wavelength.

Absorption of light in the eye

Notice that visible and near infrared light are focused onto the retina by the eye’s lens.  This makes the eye much more sensitive to injury from light in this wavelength range (400 nm – 1400 nm) because the focusing by the lens increases the power density in the laser beam.

The damage mechanisms are similar no matter where the light is absorbed.  The basic types of damage are thermal burns, photochemical reactions, and acoustic shock.  The last mechanism, acoustic shock, is caused by high-intensity short-pulse lasers when the laser light causes a sudden vaporization of tissue which then causes a shock wave to spread, tearing other tissues in the eye. Photochemical reactions either cause cloudiness in eye tissues or a loss of light sensitivity.  Near UV light that is absorbed in the lens changes proteins there causing them to become cloudy (cataracts) which blurs and dims vision. Blue light which is focused on the retina can also induce a photochemical reaction which causes the light receptors in the eye to lose sensitivity.  This is known as photo bleaching and mostly effects blue color vision.  Long exposure to bright sunlight can cause this type of vision loss.  Infrared and far UV light that is absorbed in the cornea produces an effect called photo keratitis, which can be an irritation to the eye that causes pain and watering in the eye, or it can cause some pigmentation to occur in the cornea (welder’s flash) in the case of UV light.  The effect is similar to cataracts.  Injuries to the outer parts of the eye or tearing of the eye’s back wall can be corrected by surgery (and with contact lenses.)  Photo bleaching or thermal burns to the retina cannot be healed and result in permanent loss of vision.

The Skin:

Lasers can harm the skin via photochemical or thermal burns.  Depending on the wavelength, the beam may penetrate both the epidermis and the dermis.  The epidermis is the outermost living layer of skin.  Far and Mid-ultraviolet (the actinic UV) are absorbed by the epidermis.  A sunburn (reddening and blistering) may result from short-term exposure to the beam.  UV exposure is also associated with an increased risk of developing skin cancer and premature aging (wrinkles, etc) of the skin. Longer wavelengths of light are mostly a source of thermal burns to the skin. Burns can be second or third degree, but are rarely serious because peoples reflexes cause them to move out of the way of the light.

Light Wavelengths and Injury Summary:

The part of the electromagnetic spectrum relevant to lasers is divided into seven wavelength regions as follows:

  • Ultraviolet C: 100-280 nm. Ultraviolet C radiation is responsible for photokeratitis of the cornea and erythema of the skin (sunburn).
  • Ultraviolet B: 280-315 nm. Ultraviolet B radiation is responsible for photokeratitis of the cornea, skin cancer, and accelerated aging.
  • Ultraviolet A: 315-400 nm. Ultraviolet A radiation is responsible for photochemical cataracts, pigment darkening and skin burn.
  • Visible: 400-760 nm. Visible radiation is responsible for photochemical and thermal retinal injury, photosensitive skin reactions and skin burn.
  • Infrared A: 760-1400 nm. Infrared A radiation is responsible for cataracts and retinal burns and skin burns.
  • Infrared B: 1400-3000 nm. Infrared B radiation is responsible for corneal burns, cataracts, damage to the aqueous humor, and skin burns.
  • Infrared C: 3000-106 nm. Infrared C radiation is responsible for corneal and skin burns.


Part 2: Laser Safety

Spectrum with laser emission and goggle protection ranges

Laser Power Classes:

Lasers can be divided into four basic classes according to their potential to inflict harm on the user or bystanders. 

CLASS I LASERS are low-powered and do not emit hazardous radiation under normal operating conditions because they are completely enclosed. Class I lasers are exempt from any control measures. Equipment, such as laser printers and laser disc players, are examples of this class.

CLASS II LASERS emit accessible visible laser light with power levels less than 1 mW radiant power and are capable of creating eye damage through chronic exposure. The human eye blink reflex, which occurs within 0.25 seconds of exposure to the Class II laser beam, provides adequate protection. It is possible to overcome the blink response and stare into the Class II laser long enough to damage the eye. Class II lasers are exempt from any control measures. Some visible continuous wave Helium-Neon lasers and some laser pointers, are examples of Class II lasers.

CLASS IIa LASERS are special purpose lasers that emit accessible visible laser light with power levels less than 1 mW radiant power and are not intended for viewing. This class of lasers causes injury when viewed directly for more than 1,000 seconds. Class IIa lasers are exempt from any control measures. Grocery store scanners are examples of Class IIa lasers.

CLASS IIIa LASERS are systems with power levels of 1 to 5 mW that normally would not produce a hazard if viewed for only momentary periods with the unaided eye. They pose severe eye hazards when viewed through optical instruments (e.g., microscopes, binoculars, or other collecting optics). Class IIIa lasers must be labeled. A warning label shall be placed on or near the laser in a conspicuous location and caution users to avoid staring into the beam or directing the beam toward the eye of individuals. Some visible continuous wave Helium-Neon lasers and some solid state laser pointers are examples of Class IIIa lasers.

CLASS IIIb LASERS are systems with power levels of 5 mW to 500 mW  (CW) and some pulsed lasers. These lasers will produce an eye hazard if viewed directly. This includes specular reflections.  Administrative, Engineering, and PPE control measures are required. (Especially laser safety glasses!)

CLASS IV LASERS are systems with power levels greater than 500 mW and many pulsed lasers. These lasers will produce eye, skin and fire hazards. This includes specular or diffuse reflections. Administrative, Engineering and PPE control measures are required.  (Especially laser safety glasses!)

Lasers in JRM:








532nm, 6W, CW

VIS, Class IV




800nm, 5 nJ/pulse @ 10 fs/pulse

IR-A, Class IV



Evolution 30

527 nm, 20 mJ/puls, 100 ns/pulse

VIS, Class IV




800 nm, 3 mJ/pulse @ 25 fs/pulse

IR-A, Class IV



Hollow fiber

800 nm, 1 mJ/pulse @ 6 fs/pulse





780 nm, 15 mW, CW

IR-A, Class IIIb




1529 nm, 15 mW, CW

IR-B, Class IIIb




730-820 nm, 300 mW, CW

IR-A, Class IIIb



CSU Pump

532 nm, 6W, CW

VIS, Class IV




10 μm, 50 W, CW

IR-C, class IV




780 nm, 30 mW, CW

IR-A, Class IIIb




780 nm, 350 mW, CW

IR-A, Class IIIb




780 nm, 15 mW, CW

IR-A, Class IIIb




1529 nm, 25 mW, CW

IR-B, Class IIIb




1529 nm, 25 mW, CW

IR-B, Class IIIb



Pump laser

1300 nm, 10 W, CW

IR-A, Class IV




1250 nm, 2.5 nJ/pulse @ 20-40 fs

IR-B, Class IIIb




1500 nm, 10 mW, CW

IR-B, Class IIIb




1500 nm, 500 mW, CW

IR-B, Class IIIb




1500 nm, 10 mW, CW

IR-B, Class IIIb




10 μm, 35 W, CW

IR-C, Class IV




632 nm, 8 mW, CW

VIS, Class IIIb


Personal Protective Equipment:

Personal protective equipment (PPE) is intended to protect you from hazardous exposure.  For skin exposure to laser light, the appropriate PPE is clothing that will cover any skin areas that might be exposed to laser light (long sleeves, gloves, shoes, hoods, etc.).  To avoid injury to the eyes from laser light, you need appropriate protective goggles.

The purpose of laser protective eyewear is to filter out the laser frequencies while maximizing vision from ambient light.  To select the right laser goggles, you need to know the wavelength of the radiation and what attenuation factor is needed to make the laser safe for your eyes.  Knowing the wavelength and duration of exposure, you can look up the maximum permissible exposure from the ANSI tables.  The optical density of the glasses needed is the attenuation required to reduce the power density in the beam to or below the maximum permissible exposure. 

The optical density (O.D.) of a filter is a measure of the light transmitted through the filter.  O.D. is defined as the negative log of the filter transmission (O.D. = -log10 (transmitted power/incident power)); thus an O.D. of 4 indicates that 0.01% of the incident light in the stop band of the filter will be transmitted through the filter and that 99.99% will be stopped by the filter. 

To determine the required optical density for glasses for safe operation of a laser, use the output power of the laser and the maximum permissible exposure (MPE), or exposure limit, to radiation under the laser’s operating conditions.  The required O.D. for safety glasses is given by: O.D. = log10 (laser output/exposure limit) (*note that the laser output and exposure limit must have the same units.)

Always inspect glasses for damage before wearing them. Also keep in mind that glasses can themselves be damaged by the laser exposure and lose some of their protective capacity if the power of the laser is above their damage threshold.

Another thing to be aware of when selecting laser protective eyewear is how the transmission of the goggles will effect your ambient light vision.  It is possible for the goggle to block your ability to read instruments or see controls on your apparatus.  And example would be a goggle that blocks visible red light as would be used for a HeNe laser or the KLS short-pulse beam would keep you from seeing red LED’s.  A situation like this requires the user to develop the discipline not to remove his protective glasses to read the instrument.  In cases where it is impossible to do an experiment because the laser goggles interfere too much with vision, remote monitoring of the experiment can be arranged using video cameras and monitors.

These are the laser-protective goggles that we keep in the JRML:



Optical density at wavelength




KLS long pulse,

MOT trapping,


4+ @ 755-780 nm,

7+ @ 780-840 nm,

4+ @ 840-855 nm

  Red goggles


KLS short pulse,




4+ @ 630-690 nm,

7+ @ 690-970 nm,

5+ @ 970-1100 nm,

5+ @ 10,600 nm

Blocks all visible

red light, including red LED’s

Blue goggles


KLS Short pulse,


6+ @ 690-1300

Blocks visible red LED’s



KLS + pump,

KLS 2nd harmonic,

KLS 3rd harmonic,

9+ @ 190-520 nm

7+ @ 520-532 nm

3+ @ 710-750 nm

5+ @ 750-850 nm

7+ @ 850-1080 nm

7+ @ 5-11 µm

Blocks visible blue LED’s

Brown goggles



3+ @ 850-900 nm

4+ @ 900-950 nm

5+ @ 950-1000 nm

7+ @ 1000-1600 nm

5+ @ 1600-2400 nm

5+ @ 2940-10,600 nm


Grey goggles

Administrative Controls:

A variety of warning devices have been installed in the Macdonald Laboratory to make users aware of potential hazards from laser light.  On entering the lab you will find a series of status panels just inside the door.  The panel to the far left indicates where the pulsed beam from the KLS is in use in the lab.  A map on the west wall of the control room shows the same information.  The lighted LED’s on both boards indicate where the laser is in use.  If you are going into any of those areas, you will need to have appropriate laser protective goggles.

Laser status map

Laser status map in the control room showing that KLS beam is being sent to the square-room COLTRIMS experiment.

Status board

Status board at lab entrance showing the van de Graff is in operation and that KLS light is being sent to the Square-room COLTRIMS area.

Each area where class IV lasers are in use also has an opaque barrier separating it from the rest of the lab.  The passage way through the barrier (door, gate, etc.) has on it an interlock system such that the laser will be turned off in the area if the door is opened.  These interlocks are administrative controls (not engineering controls) because they can be easily defeated without shutting down the laser system.  The purpose of the interlocks is to protect inexperienced people from laser injuries.  Someone doing an experiment with the pulsed laser has to be able to get into and out of the area without shutting down the laser, so there are bypass controls on the door interlocks.  The assumption is that if a person knows how to bypass the interlock, he will know to have put on appropriate vision protection before going into the area. The bypass is generally accomplished by pushing a button on a control box next to the interlocked passage way.  You should only bypass an interlock and go into an active laser area if you know what hazards exist in the area and you are wearing the proper personal protective equipment (laser goggles.)

KLS interlock control

Interlock control at the KLS entrance. The key pad in the center of the picture is used to enter the pass code that bypasses the door interlock to the KLS.  You can use the phone on the wall to call into the lab to talk to someone inside. The button to the right of the key pad rings a door bell if you need someone to come out to assist you.

EBIS interlock control

Interlock bypass box at the EBIS area control gate.  The blue lamp at the upper right-hand corner indicates the laser status (currently off.) Pushing the “bypass: button at the left of the box will override the interlock for a period of 90 seconds, during which the gate can be opened without interrupting the experiment in progress.

It is always the user’s responsibility to know what hazards exists in an area and to have the correct protective goggles on at all times when in the hazard zone.  Lab policy requires that all people working in a laser hazard zone be wearing correct laser protective eyewear at all times.  Failure to comply with this policy can result in the violator being barred from the lab.

Some of the controlled laser areas have moveable curtains to act as a secondary barrier (LUMOS Square-Room COLTRIMS).  These barriers are in place to keep laser light from getting out and exposing unprotected personnel when the doors are being opened and closed.  The curtains should always be kept drawn when the laser is in operation.

The KLS laser lab has an additional warning indicator to tell users which type of goggles are needed inside the lab under current operating conditions.  This display is visible above the barrier in front of the KLS main entrance.  There are two lamps on the display, one red, the other blue.  These lamps indicate is special conditions exist inside the lab.  If the RED lamp in on, the short-pulse fiber is in use, and users must wear the blue or green colored goggles that block out all of the visible red spectrum.  If the blue lamp is light, as in the picture below, the green pump laser is uncovered inside the lab and users will need to wear the brown colored goggles.  If neither light is on, then the pink NIR goggles will provide adequate protection.

Safety goggle storage

Safety goggle storage outside KLS. Different safety goggles are needed inside the KLS depending on what machines are in use. The goggles are kept sorted in the storage cabinets outside the lab so that users can easily get the appropriate eye protection.

KLS information display

Information display outside KLS. The lights tell you if special conditions exist inside the laser lab. The blue lamp shown lit indicates that users need to wear brown goggles because the pump laser is uncovered. If the red light is on, then short pulses are being generated which require the use of the blue goggles. When both lamps are off the pink goggles may be used.

Blue warning lights are turned on outside of laser areas when the laser is turned on to indicate that the laser is operating.  The blue light is one of the standard hazard warnings in the JRML. The KLS is an exception, in that the indicator lamps that the laser is on and a hazard exists inside KLS is red in color.  In all other areas, the laser warning lamp will be blue.  There are other warning lights of easily recognizable colors to indicate different hazards.  A flashing amber light is used to indicate that a shock hazard exists from high voltage. Red strobe lights are used to warn you that penetrating radiation is present in an area.  The other color of warning light is white.  The white strobe is always used to indicate that an emergency condition exists and that you should leave the area immediately.  These strobes are installed on the fire alarms and on the low oxygen level alarm.  Whenever you see a flashing white light (generally with an accompanying audible buzzer) you should leave the lab immediately and contact technical staff from upstairs. Do not try to investigate a fire or low oxygen condition by yourself!

High voltage warning light

High voltage cage at EBIS with amber warning light.

LUMOS laser warning light

Entrance to LUMOS lab. The blue light outside the door indicates that the IR lasers are operating inside the lab.  Safety goggles for the IR-B band are stored in the cabinet below the warning light.

Oxygen deprivation alarm strobe

White strobe warning for low oxygen level. Personnel should leave the area immediately in case of white strobes, which either indicate a fire alarm or low oxygen levels.

LINAC radiation warning

View of LINAC common beam line showing high radiation level alert.  All personnel should leave the area when this light is on.


Engineering Controls:

Certain of the class IV lasers in use in the JRML are enclosed so that personnel are not exposed to the beams.  The enclosures have interlocks in place which shut down the laser any time the enclosure is opened.  Examples are the KLS pump lasers and the CSU CO2 laser.  It is not necessary to wear protective eye wear for these lasers under normal operating conditions, since beam exposure is prevented.  If the interlocks have been defeated so that enclosure can be opened while the laser is operated, as when tuning the beam, then a sign will be posted in the area to warn personnel of the hazard.

Ionizing radiation:

Some of the personnel control gates in the JRML are to protect people from multiple types of hazards.  When the hazard in a controlled area is laser light, proper personal protective equipment (laser goggles) will protect users from the hazard.  I this case it is acceptable to bypass gate interlocks and go into the area if you are wearing the proper PPE.  When the hazard is penetrating radiation, such as neutrons or X-rays, there is no protective equipment you can wear to keep you safe from the radiation.  Areas where there are high levels of penetrating radiation are, therefore, off limits to all personnel.  Control gates for high radiation areas, have interlocks which cannot be bypassed.  The interlocks on these gates will shut down the van de Graff accelerator if the gate is opened, which also shuts down the experiment that is in progress.

Control gate Status lights

The control gate between the LINAC hall and the ECR (left) can be shut either for laser hazards or for penetrating radiation generated by ion beams.  The “beam on” light shown at right lets you know if ion beam is going into the LINAC area. You still need to check the radiation monitor panel in the control room to see if there is a radiation hazard.

When a lab user comes to a closed control gate, it is the users responsibility to know what hazard is beyond the gate.  If the hazard in the controlled area is penetrating radiation, then you should not eneter the area.  You will need to contact the accelerator operator and find out when the experiment can be conveniently interrupted so that the radiation source can be shut off and you can gain safe access to the area.  To determine if penetrating radiation is present in a lab area, you can check the area radiation monitors in the control room.  The panel shows the radiation dose rate at each of the monitoring stations around the lab.  There is also a map to show you where each station is located.  The map also shows the locations of the control gates.  A red LED in a gate location indicates that there is no radiation interlock active on that gate.  If the LED is green in color, then the interlock as been set so that opening the gate will shut down the system.  You should not open a gate with a green LED without first informing the person running the experiment.

Radiation monitors

Radiation monitor display in the control room. Each readout is the radiation dose rate at one of the area monitoring detectors in the lab.  The map at lower right shows the locations of the detectors and the location and status of personnel control gates. A red LED on the map indicates there is no interlock for radiation on the gate.  A green LED indicates an active gate interlock tied to the van de Graff accelerator.

Standard Operating Procedures:

Engineering and Administrative controls cannot protect you from all laser-related injuries. It is important that everyone observe safe practices whenever they are working with high-powered lasers. Furthermore, you are not only responsible for your own safety, but whenever you are working with high-power lasers, you must also avoid creating hazards for other people working in the area. The following procedures will help to protect you and others from laser accidents.

  • Always wear personal protective equipment when working around Class IV lasers, or changing the beam path of class IIIb lasers. PPE includes proper eye protection as well as protection for the skin when relevant.
  • Remove metal/reflective jewelry from hands and wrists when working around laser beams. Rings, watches and bracelets can all produce specular reflections of laser beams tarveling in random directions. It is best to remove all such items from your hands before going into any laser lab.
  • Never look along a laser line towards the radiation source.
  • View laser beams using imaging cards that allow safe viewing through protective goggles. Never remove your goggles to view a class IV laser directly on a diffuse surface.
  • If a viewing card has a smooth surface, always position the card so that any surface reflections will go in a safe direction (towards the table, for example.)
  • Block the incident beam before changing any laser transport optic.
  • After altering a laser beam transport optic, place a beam block downstream of the optic before allowing laser light into the system. Then trace the beam all the way from the new optic through the transport path to make sure no stray beams are crossing user areas.
  • Flipper mounts or other moving optics mounts should be arranged so that transient beams reflected during changing the mount position will go in a safe direction. For example, a standard flipper mount should be placed so that it flips downward toward the incident radiation. This way, surface reflections will go downward toward the table when the mount is changed from one state to the other. The mount should never be placed so that the transient beams can go upward toward the operatorís face.
  • Even when a flipper is correctly mounted, the incident beam should be blocked before the mount is flipped from one state to the other!
Laser optics flipper mount practices

Flipper mounts should reflect the beam in a safe direction when moved; even then, the incident beam should be blocked before the mount is flipped.


Part 3: Lab Safety

Compressed Gas Safety

Hazards from compressed gases:

  1. Cylinder weight: A cylinder that falls over can break equipment or injure personnel.  Cylinders need to be secured at all times so that they can not fall over.
  2. Gas volume:  A full cylinder can contain 5,000 liters of gas. A sudden discharge of the gas would displace air from the work area. Thus, even inert gases like helium or argon represent asphyxiation hazards if the gas is released into a closed space.
  3. Gas hazards: Gases in cylinders can be toxic or flammable.  In the Macdonald lab, we work with CO, which is deadly at a concentration of 100 p.p.m.  Accidental discharge of even a small amount of such gas into a closed room would present a serious health hazard.  We also have flammable or explosive gases like hydrogen, methane, and acetelene.  These gases need to be kept away from sparks or flames.  A special hazard is silane, which burns spontaneously in contact with air.  Special measures are needed to purge silane from gas manifolds so that it does not cause a fire.
  4. Cylinder energy: considerable mechanical energy is stored in the compressed gas in a cylinder. If an accident breaches the cylinder valve, that mechanical energy is released as thrust.  The cylinder can accelerate to speeds great enough to penetrate concrete walls.  The picture below shows a hole in a concrete floor made by a compressed gas cylinder (actually a liquid nitrogen dewar that was allowed to build up too much pressure) that ruptured in use.
Gas cylinder accident results

Hole in a concrete floor that was mad by a gas storage cylinder (cryogenic) that ruptured its bottom and jetted upward through the ceiling and into the room above. The gas discharge also blew out the walls of the lab where the tank was stored.

Compressed gas Standard Operating Procedures:

  1. Storage of cylinders
    1. Cylinders should be secured to a wall or other structure so that they cannot fall over.
    2. A cylinder that is not in use should have the regulator removed and the safety cap installed over the valve.
    3. If a cylinder is empty, it should have a ring put over the safety cap to mark it as empty or be removed from the lab.
  2. Moving a gas cylinder
    1. Always remove the regulator and install a safety cap before moving a gas cylinder.
    2. Transport a cylinder on a four-wheeled cylinder cart with the chain secured so that the cylinder cannot fall off the cart.
  3. Connecting a gas cylinder to an experiment
    1. Always secure the cylinder to a wall bracket or other support structure before removing the safety cap.
    2. Check that the regulator sealing surface and the connector threads are clean and undamaged.  If a gasket is needed, make sure it is in good condition.  Make sure the regulator is appropriate for the gas you are using.
    3. Tighten the regulator to the cylinder using a wrench.  Most cylinder nuts are 1 1/8 inch size.  There are box wrenches in the lab for this size, or you can use an adjustable wrench.  Most nuts tighten by turning the nut clockwise when looking from the regulator towards the cylinder.  Left-hand nuts are marked with a grove around their middle, and tighten the other way.
    4. Check that the connections to the gas line going to the regulator are not damaged and attach the gas line.
    5. The procedure for purging air from the regulator and delivery line will depend on the type of gas and the regulator.
      1. Some regulators are tagged as being “for vacuum service”.  With these regulators, the whole gas delivery system can be pumped out all the way to the cylinder valve.
      2. For non vacuum service regulators, air is purged by flowing gas through the system
      3. If the gas is toxic, it cannot be purged into the lab.  Contact lab technical staff in this case.
    6. Check for leaks in the system after all connections are tightened.  This is usually done by opening the valve on top of the cylinder to pressurize the regulator.  Then the cylinder valve is closed, and the pressure in the regulator is monitored to see if it leaks down.  If the gas is toxic, have lab technical staff install and check the gas bottle.
  4. Using compressed gas
    1. Before opening the valve on a gas cylinder, always make sure that the regulator is shut off.  To do this turn the handle in the center of the regulator bonnet fully counter clockwise, either until it stops or until all spring tension on the shaft is released.
    2. Open the valve on the cylinder slowly by turning it counter clockwise.  Watch the pressure gauge on the inlet side of the regulator to know when the valve is open.
    3. Set the desired operating pressure coming out of the regulator by turning the regulator control clockwise while watching the output pressure gauge.
    4. When you are finished with your experiment, close the valve on the gas cylinder and turn the regulator pressure control fully counter clockwise.
  5. Removing a gas cylinder from use
    1. Be sure the valve on the cylinder is closed (clockwise) and that the regulator pressure control is set at its minimum (counter clockwise).
    2. If the gas is toxic, it must be purged from the regulator and lines before anything can be opened to air.  Contact lab technical staff for help.
    3. Remove the delivery line from the regulator.
    4. Remove the regulator from the gas bottle.  Generally this is done by turning the nut on the regulator adapter counter clockwise.  Some regulators have left-handed threads on them.  These are marked by a grove around the flats of the nut.  Turn left-hand nuts clockwise to loosen them.
    5. Screw the safety cap onto the cylinder.  If the cylinder is empty, place a ring over the safety cap.
Compressed gas storage

Compressed gas cylinder storage in the Macdonald Laboratory:

Cylinders are kept chained to the wall so that they cannot fall over.  The safety caps must be secured over the valve of the cylinder anytime a gas bottle is moved or taken out of service.

The black cylinder at the back right has a yellow ring placed over the cap of the bottle to indicated that the cylinder has been emptied of gas.

The cylinder to the far right is in a locked storage area that is reserved for toxic gases (the cylinder contains carbon monoxide.)  If you need a gas from the locked storage areas, you must contact lab technical staff for setup of the cylinder.

Compressed gas usage

Gas cylinders in use:

These cylinders are all in use at the diode source. The regulators work the same way irrespective of the type of gas they are installed on. Turning the knob or handle in the center of the regulator bonnet clockwise will increase the pressure in the gas delivered to the output line. The pressure control should always be turned fully counter clockwise before the valve to the cylinder is opened. It is also recommended when you finish an experimental run, that you shut off the regulator by backing out the pressure control counter clockwise.

The valves on the bottle always open by turning them counter clockwise as viewed from above.

The pressure gauge nearest the cylinder shows the pressure in the cylinder, and the gauge farthest from the bottle is the pressure coming out of the regulator.

Compressed gas connections

Gas cylinder connections:

Different kinds of gases have different fittings at the cylinder valves where the regulator screws on. The nut on the regulator shown to the left has left-handed threads, as indicated by the notch made around the nut flats. To remove such a regulator from a gas cylinder, you must turn the nut clockwise when you are looking from the regulator towards the cylinder.

The most common lab gasses with left-hand threaded fittings are hydrogen, sulfur hexafluoride, methane, silane, deuterium, and air.

The regulator works in the normal fashion. Turning the control knob clockwise increases the output pressure and turning it counter clockwise decreases the output pressure.

The gauges are in units of “pounds per square inch” (psi)

Last updated on Thursday, 08-Jan-2015